Solution conformation dynamics of a tetrasaccharide related to the Lewisx antigen deduced by 1H NMR NOESY, ROESY, and T-ROESY measurements

The conformational and dynamical features of a Lex tetrasaccharide analogue GaINAc(α 1–3)Gal(β 1–4)[Fuc(α 1–3)]Glc(βOMe) 1 have been studied through 1H NMR relaxation measurements. The results indicate that the different glycosidic linkages of 1 present distinct conformational flexibility in solution. In addition, the use of T-ROESY experiments in conformational analysis of oligosaccharides is explored emphasizing its scope and limitations. The conformational and dynamical features of a tetrasaccharide. GalNac(α1–3)Gal(β1–4)[Fuc(α1–3)]Glc(βOME) 1 are analysed via 1H NMR relaxation measurements.