(4-O-Methyl-α-d-glucurono)-d-xylan from Rudbeckia fulgida, var. sullivantii (Boynton et Beadle)

From the medicinal plant Rudbeckia fulgida, var. sullivantii (Boynton et Beadle) a low-molecular-mass (4-O-methyl-α-d-glucurono)-d-xylan was isolated by alkaline extraction, followed by ethanol precipitation, ion-exchange chromatography, and gel filtration. The results of compositional and linkage analyses, supported by those of 1H and 13C NMR measurements of oligomers generated on partial acid hydrolysis, showed the (1→4)-linked β-d-xylopyranosyl backbone with about 18% of 4-O-methyl-d-glucuronic acid attached to O-2 of the xylose residues. From the mean distance of adjacent carboxyl groups, obtained from experimentally determined single-ion activity coefficients of calcium counterions, it followed that the uronic acid units are separated and distributed regularly along the xylan chain, i.e. approximately each sixth d-xylose unit bears a 4-O-methyl-d-glucuronic acid residue.