Isolation and characterization of di- and tri-mannosyl-cyclomaltoheptaoses (β-cyclodextrins) produced by reverse action of α-mannosidase from jack bean

Di- and tri-mannosyl-cyclomaltoheptaoses (β-cyclodextrins, βCDs), which were synthesized together with mono-mannosyl-βCD in a large-scale production by reverse action of α-mannosidase from jack bean, were isolated and purified by HPLC. The structures of seven isomers of di-mannosyl-βCD and six isomers of tri-mannosyl-βCD were elucidated by FABMS and NMR spectroscopy, and by enzymatic methods.