Effects of various saccharides on cycloinulo-oligosaccharide fructanotransferase reaction: production of β-inulotriosyl-α-d-mannopyranoside and 1-O-β-inulotriosyl-α-l-sorbopyranose

The effects of various saccharides on the reaction of cycloinulo-oligosaccharide fructanotransferase with cycloinulohexaose were examined. In addition to β-d-fructofuranosides and methyl α-d-glucopyranoside, d-mannose and l-sorbose were found to be effective acceptors in the reactions, and they enhanced the hydrolytic activity as effectively as methyl α-d-glucopyranoside. Hetero-tetrasaccharides were isolated as the major transfer products from both reaction mixtures. The isolates were identified by NMR spectroscopy as β-inulotriosyl-α-d-mannopyranoside and 1-O-β-inulotriosyl-α-l-sorbopyranose. Methyl β-d-glucopyranoside was slightly effective and methyl α-d-mannopyranoside was not effective at all as the acceptor, but these saccharides strongly enhanced the hydrolytic activity. d-Glucosamine inhibited the enzyme activity.