Size-exclusion chromatography study of the molecular-weight distribution of γ-irradiated pullulan Original Research Article

The polyelectrolyte behavior of γ-irradiated pullulan in aqueous solutions leads to secondary effects of adsorption on a Shodex OHPak KB 806 column gel during size-exclusion chromatography. Suppression of the polyelectrolyte properties of γ-irradiated pullulan is achieved by using a 0.05 M aqueous solution of NaH2PO4 (pH 4.95) as the mobile phase. Under these conditions, adequate molecular-weight distributions of γ-irradiated pullulan samples are obtained.