Double oxidation of d-xylose to d-glycero-pentos-2,3-diulose (2,3-diketo-d-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus

Pyranose dehydrogenase purified to homogeneity from the mycelia of the basidiomycete fungus Agaricus bisporus catalyzed the oxidation of d-xylose at C-2 to d-threo-pentos-2-ulose (2-keto-d-xylose) and successively at C-3 to d-glycero-pentos-2,3-diulose (2,3-diketo-d-xylose) using 1,4-benzoquinone as an electron acceptor. The sites of oxidation were deduced from the spectroscopic analysis (MS, NMR) of the N,N-diphenylhydrazone derivatives of the reaction products.