Title of article
A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa-carrageenase
Author/Authors
Svein H. Knutsen، نويسنده , , Marit Sletmoen، نويسنده , , Trond Kristensen، نويسنده , , Tristan Barbeyron، نويسنده , , Bernard Kloareg، نويسنده , , Philippe Potin، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2001
Pages
6
From page
101
To page
106
Abstract
Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosaccharides. It was used to evaluate the elution profiles of hydrolysates produced by carrageenases specific for kappa- and iota-carrageenans. Oligosaccharide peaks ranging from di- to dodeca-saccharides were obtained in about 20 min on an analytical scale, whereas preparative runs were completed in a few hours. The method may also be used to monitor polysaccharide degradation.
Keywords
Iota-carrageenase , Sulphated neocarrabiose oligosaccharides , Kappa-carrageenase , Carrageenan , size-exclusion chromatography , NMR
Journal title
Carbohydrate Research
Serial Year
2001
Journal title
Carbohydrate Research
Record number
963146
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