Title of article
Efficient chemoenzymatic synthesis of globotriose and its derivatives with a recombinant α-(1→4)-galactosyltransferase Original Research Article
Author/Authors
Jianbo Zhang، نويسنده , , Przemyslaw Kowal، نويسنده , , Jianwen Fang، نويسنده , , Peter Andreana، نويسنده , , Peng George Wang، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2002
Pages
8
From page
969
To page
976
Abstract
A truncated α-(1→4)-galactosyltransferase (LgtC) gene from Neisseria meningitidis was cloned. The recombinant glycosyltransferase was expressed in Escherichia coli BL21 (DE3) strain with high specific activity (5 units/mg protein). Its acceptor specificity was carefully characterized. Then the purified enzyme was utilized in highly efficient syntheses of globotriose and a variety of α-(1→4)-galactosylated derivatives as potential antibacterial agents.
Keywords
Globotriose , Chemoenzymatic synthesis , LgtC , ?-(1?4)-galactosyltransferase , Recombinant enzyme
Journal title
Carbohydrate Research
Serial Year
2002
Journal title
Carbohydrate Research
Record number
963480
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