Structural determination of the complex exopolysaccharide from the virulent strain of Cryphonectria parasitica Original Research Article

The structure of a new exopolysaccharide from the virulent strain of Cryphonectria parasitica was elucidated by means of 2D NMR spectroscopy and selective degradations (mild hydrolysis and acetolysis). The polysaccharide is built up of mannose, galactose and rhamnose and has a rather complex non-repetitive structure that can be idealised as follows: Article Outline 1. Introduction 2. Results and discussion 3. Conclusions 4. Experimental 4.1. Fungal cultures 4.2. Purification of EPS 4.3. NMR spectroscopy 4.4. Gas chromatography 4.5. Compositional and methylation analysis 4.6. Mild hydrolysis 4.7. Acetolysis of EPS I Acknowledgements References