A new mannoheptaose containing α and β-(1→2) linkages isolated from the mannan of Torulaspora delbrueckii: ELISA inhibition studies Original Research Article

Torulaspora delbrueckii starin IFO 0955 was examined with respect to its structural and serological properties of the cell wall mannan (Td-0955-M). Td-0955-M revealed significant reactivities with sera from a commercially available factor serum kit (Candida Check) in ELISA. Td-0955-M was investigated for its chemical structure by acetolysis under conventional and mild conditions. NMR and GC techniques were used as analytical techniques. The mannooligosaccharide fractions eluted from a Bio-Gel P-2 column were found to consist of Man(α1-2)Man, M2, Man(α1-2)Man(α1-2)Man and Man(β1-2)Man(α1-2)Man, M3, Man(α1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M5, and a new mannoheptaose, which possesses the structure, Man(α1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M7. The results of the inhibition ELISA showed that the M7 oligosaccharide significantly inhibited the reactivities in the Td-0955-M-factor serum systems.