Structure of the O-polysaccharide of a serologically separate strain of Proteus mirabilis, TG 332, from a new proposed Proteus serogroup O50 Original Research Article

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis TG 332 strain. The following structure of the O-polysaccharide was determined by chemical methods along with NMR spectroscopy, including 2D COSY, TOCSY, ROESY and 1H, 13C HMQC experiments: Article Outline 1. Introduction 2. Results and discussion 3. Experimental 3.1. Bacterial strain, growth, and isolation of the lipopolysaccharide 3.2. Degradation of the lipopolysaccharide 3.3. Monosaccharide analysis 3.4. Methylation analysis 3.5. NMR spectroscopy 3.6. Rabbit antiserum and serological assays Acknowledgements References