Structural and immunochemical relationship between the O-antigenic polysaccharides from the enteroaggregative Escherichia coli strain 396/C-1 and Escherichia coli O126 Original Research Article

The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 396/C-1 has been determined. Sugar and methylation analyses together with 1H and 13C NMR spectroscopy were the main methods used. Inter-residue correlations were determined by 1H,1H-NOESY, 1H, Article Outline 1. Introduction 2. Results and discussion 3. Experimental 3.1. Bacterial strain and conditions of growth 3.2. Preparation of lipopolysaccharide and lipid-free polysaccharide 3.3. Immunochemical analyses 3.4. Component analyses 3.5. GLC and GLC–MS analyses 3.6. NMR spectroscopy Acknowledgements References