Synthesis of 2-[(2-pyridyl)amino]ethyl β-d-lactosaminide and evaluation of its acceptor ability for sialyltransferase: a comparison with 4-methylumbelliferyl and dansyl β-d-lactosaminide Original Research Article

We reported the synthesis of β-d-lactosaminide with a 2-aminopyridyl group that is linked to a glycosyl tether at the reducing end. This fluorescent disaccharide acts as an acceptor for both α-(2 → 6)- and α-(2 → 3)-sialyltransferases. In addition, the acceptor ability of this disaccharide was evaluated and compared with that of β-d-lactosaminide having a dansyl or a 4-methylumbelliferyl group.