Enzyme-catalysed synthesis of galactosylated 1d- and 1l-chiro-inositol, 1d-pinitol, myo-inositol and selected derivatives using the β-galactosidase from the thermophile Thermoanaerobacter sp. strain TP6-B1 Original Research Article

The products from the enzymatic β-d-galactopyranosylation of 1d-chiro-inositol, 1d-pinitol, 1d-3-O-allyl-4-O-methyl-chiro-inositol, 1d-3,4-di-O-methyl-chiro-inositol, 1l-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the β-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl β-d-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.