Structure of minor oligosaccharides from the lipopolysaccharide fraction from Pseudomonas stutzeri OX1 Original Research Article

A minor oligosaccharide fraction was isolated after complete de-acylation of the lipooligosaccharide extracted from Pseudomonas stutzeri OX1. The full structure of this oligosaccharide was obtained by chemical degradation, NMR spectroscopy and MALDI-TOF MS spectrometry. These experiments showed the presence of two novel oligosaccharides (OS1 and OS2): where R = (S)-Pyr(→4,6) in OS1 and α-Rha-(1→3) in OS2. All sugars are d-pyranoses, except Rha, which is l-pyranose. Hep is l-glycero-d-manno-heptose, Kdo is 3-deoxy-d-manno-oct-2-ulosonic acid, Pyr is pyruvic acid, P is phosphate.