Structure elucidation by NMR spectroscopy of a new acetylated saponin from Centratherum anthelminticum Original Research Article

A new glycosylated triterpene has been isolated from the seeds of Centratherum anthelminticum, a medicinally important plant. The structural analysis of its acetylated derivative was performed by 1H, 13C NMR, 1H–1H COSY, HMQC, HMBC and DEPT spectroscopy. The saponin was shown to contain hederagenin and six sugar residues forming two glycosyl chains. The complete structure of the saponin was established as 3-O-[β-d-glucopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→2)-α-l-arabinopyranosyl]-28-O-[β-d-glucuronopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→3)-β-d-glucopyranosyl]-hederagenin.