Title of article
Structures of two polysaccharides of Campylobacter jejuni 81116 Original Research Article
Author/Authors
Jimmy Muldoon، نويسنده , , Alexander S. Shashkov، نويسنده , , Anthony P. Moran، نويسنده , , John A. Ferris، نويسنده , , Sofʹya N. Senchenkova، نويسنده , , Angela V. Savage، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2002
Pages
7
From page
2223
To page
2229
Abstract
Campylobacter jejuni 81116 has been extensively investigated in studies on genes associated with the synthesis of Campylobacter lipopoly/lipooligosaccharides (LPS/LOS). Despite these investigations, data on the chemical structure of polysaccharides from C. jejuni 81116 have been absent. The present study was undertaken to fill that void. Biomass was grown in large quantities on agar medium, harvested and extracted by hot phenol–water extraction. Subsequently, extracts were treated by DNase, RNase and proteinase K to remove contaminants. After mild acid treatment, followed by preparative gel-permeation and anion-exchange chromatography, fractions were isolated and studied by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, 1H,13C HMQC and HMBC experiments. These advanced investigations
Keywords
Polysaccharide structure , NMR abstract , Campylobacter jejuni
Journal title
Carbohydrate Research
Serial Year
2002
Journal title
Carbohydrate Research
Record number
964613
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