In vitro sulfation of N-acetyllactosaminide by soluble recombinant human β-Gal-3′-sulfotransferase

Membrane-bound β-Gal-3′-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm–Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of β-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.