Structural analysis of a novel saccharide isolated from fermented beverage of plant extract

Fermented beverage of plant extract was prepared from about 50 kinds of vegetables and fruits. Natural fermentation was carried out mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp. and Pichia spp.). Three kinds of saccharides have been found in this beverage and produced by fermentation. The saccharides isolated from the beverage using carbon-Celite column chromatography and preparative HPLC, were identified as a new saccharide, β-d-fructopyranosyl-(2→6)-d-glucopyranose, laminaribiose and maltose by examination of constituted sugars, GLC and GC–MS analyses of methyl derivatives and MALDI-TOF-MS and NMR measurements of the saccharides.