Author/Authors :
David Soriano del Amo، نويسنده , , Wei Wang، نويسنده , , Christen Besanceney، نويسنده , , Tianqing Zheng، نويسنده , , Yizheng Fan، نويسنده , , Brian Gerwe، نويسنده , , Ronald D. Seidel III، نويسنده , , Peng Wu، نويسنده ,
Abstract :
A combination of recombinant FKP and α-(1→3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5′-diphosphate-β-l-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.
Keywords :
Chemoenzymatic synthesis , Transferases , Sialic acid , Fucose
