Title of article :
Structural and genetic characterization of the O-antigen of Escherichia coli O161 containing a derivative of a higher acidic diamino sugar, legionaminic acid Original Research Article
Author/Authors :
Xiaomin Li، نويسنده , , Andrei V. Perepelov، نويسنده , , Quan Wang، نويسنده , , Sof’ya N. Senchenkova، نويسنده , , Bin Liu، نويسنده , , Sergei D. Shevelev، نويسنده , , Xi-Guo Lee، نويسنده , , Alexander S. Shashkov، نويسنده , , Wei Chen، نويسنده , , Lei Wang، نويسنده , , Yuriy A. Knirel، نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2010
Abstract :
The O-antigen is an essential component of lipopolysaccharide on the surface of Gram-negative bacteria and plays an important role in its pathogenicity. Composition and structure of the O-antigens of Escherichia coli are highly diverse mainly due to genetic variations in the O-antigen gene cluster. In this work, the chemical structure and the gene cluster of the O-antigen of E. coli O161 were studied. Chemical degradations, sugar analyses, and NMR spectroscopy showed that the O161 antigen possesses a trisaccharide O-repeating unit containing a 5-N-acetyl-7-N-(d-alanyl) derivative of 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid (Leg5Ac7Ala) and having the following structure:
Keywords :
Escherichia coli , O-polysaccharide structure , O-Antigen gene cluster , 5 , Legionaminic acid , 7-Diamino-3 , Lipopolysaccharide , 5 , 9-tetradeoxynon-2-ulosonic acid , 7
Journal title :
Carbohydrate Research
Journal title :
Carbohydrate Research
