Structural analyses of the core oligosaccharide from the lipopolysaccharide of bovine and ovine strains of Mannheimia haemolytica serotype 2

Previous structural studies in our laboratory on lipopolysaccharide derived core oligosaccharide had identified a conserved inner core structure in several strains of the veterinary pathogens Mannheimia haemolytica, Actinobacillus pleuropneumoniae and Pasteurella multocida. In this study we describe the elucidation of the core oligosaccharide structure of two strains from M. haemolytica serotype 2. Structural information was established by a combination of monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structure for the core oligosaccharide was determined on the basis of the combined data from these experiments: