Validation of a method for the detection of E. coli O157:H7 in foods

This paper describes the results of a collaborative trial for the validation of a method for the detection of E. coli O157:H7 in foods. The design, sample preparation and results of the trial are discussed. An optimised laboratory protocol is proposed for improving the recovery of E. coli O157 from a range of foods which can be used as a reference method for the purposes of Official Food Control and against which other evolving methodologies can be evaluated. This protocol has also been accepted as a draft International Standard by the International Organisation for Standardisation.