A method of distinguishing human and bovine milk samples based on soluble phosphate content

The present study provides a rapid method for distinguishing human and bovine milk samples. The method is based on quantifying soluble phosphate content in milk. Sahiwal cows’ milk was closer to human mothers’ milk, due to its significantly lower soluble phosphate level, compared to the milk from Holstein cow and buffalo. Therefore, when human mothers’ milk is not available, among the bovine milk, Sahiwal cows’ milk may be a better alternative, then with from the Holstein cow or buffalo, for the preparation of infant formula. The method may be useful for quality control of commercially available milk samples for preparing infant formulation.