Simultaneous detection of norovirus and rotavirus in oysters by multiplex RT–PCR

Multiplex reverse transcription–polymerase chain reaction (RT–PCR) was used to detect noroviruses (NVs) and rotaviruses (RVs) in artificially and naturally contaminated oysters. Clearly expected amplicons of 327 bp from NVs and 392 bp from RVs were obtained, respectively. The sensitivity was 200 pg RNA/g oyster tissue. In a total of 150 naturally contaminated oysters that were highly polluted by feces, 5 (3.33%) were rotavirus, 21 (14%) were norovirus GII and 6 (4%) were norovirus GI. The results clearly demonstrate that the multiplex RT–PCR method is a specific, sensitive and time-saving method and can be used to detect NVs and RVs in marine products before their entering the markets.