Title of article
Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR
Author/Authors
Belgin Sarimehmetoglu، نويسنده , , Mihriban Hatun Aksoy، نويسنده , , Naim Deniz Ayaz، نويسنده , , Yildiz Ayaz، نويسنده , , Ozlem Kuplulu، نويسنده , , Yusuf Ziya Kaplan، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2009
Pages
5
From page
357
To page
361
Abstract
In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with E. coli O157. By PCR analyse in two of the isolates fliCh7 gene was detected and identified as E. coli O157:H7. According to the multiplex PCR one of the isolate has all stx1, stx2, eaeA, hly and fliCh7 genes and the other has stx1, eaeA, hly and fliCh7 genes.
Keywords
Multiplex PCR , Ground beef , E. coli O157:H7
Journal title
Food Control
Serial Year
2009
Journal title
Food Control
Record number
976305
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