Title of article
Evaluation of DNA extraction procedures for traceability of various tomato products
Author/Authors
Manuela Turci، نويسنده , , Maria Luisa Savo Sardaro، نويسنده , , Giovanna Visioli، نويسنده , , Elena Maestri، نويسنده , , Marta Marmiroli، نويسنده , , Nelson Marmiroli، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2010
Pages
7
From page
143
To page
149
Abstract
Globalization of food trade requires the development of integrated approaches, such as traceability of origin, quality and authenticity, to ensure food safety and consumers satisfaction. In this study, different genomic DNA extraction procedures were evaluated for their applicability to internal traceability of different products in the tomato food chain. Quality, quantity and amplificability by SSR markers of extracted DNA tallied the methods performances; times and costs were considered too. The results were processed with “fuzzy-logic” approach. “Wizard” (Promega) scored the best performance in methods final ranking. This work demonstrated the value of genomic methodologies for internal traceability of tomato-derived goods.
Keywords
DNA extraction methods , Fluorimetric analysis , Solanum lycopersicum L. , Fuzzy logic , SSR markers , PCR amplification
Journal title
Food Control
Serial Year
2010
Journal title
Food Control
Record number
976466
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