Characteristics of enzymatically-deesterified pectin gels produced in the presence of monovalent ionic salts Pages 1926-1929 S.-H. Yoo, B.-H. Lee, B.J. Savary, S. Lee, H.G. Lee, A.T. Hotchkiss Show preview | Purchase PDF - $41.95 | Recommended ar

Pectin methylesterases (PMEs) from Valencia orange (p-PME) and Aspergillus aculeatus (f-PME) were used to produce pectin gels in the presence of 0.2 M monovalent ionic salts. At pH 5.0, pectin gels were induced following enzymatic deesterification of high methoxy pectin, with greater deesterification observed using p-PME compared to f-PME. Under these conditions, the deesterification limit of f-PME ended up with a pectin of DE 30.5–31.9 which did not gel at the PME reaction completion, while p-PME reduced the pectinʹs DE to 16.0–17.2, resulting in gel formation. The pectin gel induced by KCl was significantly stronger than the NaCl-induced gel, but LiCl did not induce pectin gelation. The gel strength was influenced by both DE and species of monovalent cation. The KCl-induced gels released less water than NaCl-induced gels. A synergistic effect on gel strength was observed from the pectin treated with a combination of (p + f)-PMEs, producing even more stable gels. These results indicated that the pectin gelation of our system would be enhanced both by using larger monovalent cation and by lowering the DE value, which would presumably be attributed to the different action patterns recognized for p- and f-PMEs. This pectin gelation system could provide a useful alternative to acid-sugar or calcium cross-linked gels in food and other industrial applications.