Effect of lung extract growth factors on the differentiation of umbilical cord stem cells into blood cells in vitro in adult mouse

There are many diseases related to disorganized blood cells, especially erythrocytes and granulocytes. Therefore, it is important to find some methods for producing them. In this research work, we used extract of mouse lung to investigate the effects of its hematopoietic growth factors on stem cell differentiation in vitro. Stem cells were isolated from umbilical cord by enzyme digestion and cultured in an appropriate culture medium. In this study, four test groups were considered including experimental groups 1 & 2 (E1 & E2), which were exposed to 50% and 70% concentration of lung extract for 7 days, respectively, the sham (Sh) group, which was not exposed to lung extract, and the control (C) group, which were the same volume of mouse blood. E and Sh groups were incubated for 7 days. Mentioned groups were marked with alkaline phosphatase detection kit, their cells were counted and hematopoietic factors were measured. Morphological, cytological and differential examinations showed significant changes in E2 group as compared to the Sh group. E2 cells were differentiated into erythroid and myeloid lineages. These findings suggest that growth factors in lung extract (especially in E2) have progressive effects on umbilical cord stem cell differentiation into blood cells.