Author/Authors :
Maliji، Ghorban Ghorban نويسنده Cellular and Molecular Biology Research Center (CMBRC), Babol Universityof Medical Sciences, Babol, Iran Maliji, Ghorban Ghorban , Jafari، Sina Sina نويسنده Student Research Committee, Babol University of Medical Sciences, Babol, Iran Jafari, Sina Sina , Azadmehr، Abbas نويسنده Immunology Department, Qazvin University of Medical Sciences, Qazvin, Iran. , , Moosavi، Seyed Ehsan نويسنده Department of immunology and microbiology, Babol University of Medical Sciences, Babol, Iran. Moosavi, Seyed Ehsan , Taheri، Ebad نويسنده Dental School, University of Medical Sciences, Babol, Iran. Taheri, Ebad , Maliji، Ehsan نويسنده Student research committee, dental school, University of Medical Sciences, Babol, Iran. Maliji, Ehsan
Abstract :
Background and Aim: Periodontitis is an inflammatory disease of toothsupporting
tissues; several factors are involved in the development and severity of
periodontitis among which smoking can be mentioned; however, the exact
mechanism of the effect of smoking on progression of periodontitis is not still well
known. In this regard, the present study was conducted to evaluate Interleukin-10
and gamma interferon levels in gingival crevicular fluid (GCF) of patients with
chronic periodontitis.
Methods: This case-control study was carried out on 60 men referred to the
Department of Periodontology of Babol Faculty of Dentistry; 30 smokers and 30
age-matched non-smokers, both with chronic periodontitis, entered the case and
the control group respectively. Those with systemic disease were excluded from
the study. Assessment of periodontal health was performed by using the dental
plaque, Barnett gingival bleeding and probing pocket depth (PPD) indices.
Cytokines level in the GCF evaluated by Enzyme linked Immunosorbent Assay
(ELISA). Data were analyzed by SPSS18 statistical software by using Mann-
Whitney and Spearman rho tests.
Results: The mean dental plaque index showed no significant difference between
the two groups (P=0.1). Although gingival bleeding index was higher in control
compared to the case group, the difference was not significant (P=0.08). The mean
PPD was lower in the case that the control group (P=0.02). The mean Interleukin-
10 and gamma interferon levels was respectively 1.25 (±0.04) pg/ml and 0.82
(±0.44) pg/ml in smoking and 1.22 (±0.44) pg/ml and 0.75 (±0.32) pg/ml in nonsmoking
group (P > 0.05). In addition, a reverse correlation has been found
between gamma interferon and Interleukin-10 in both groups which was not
significant (P > 0.05).
Conclusion: IL-10 level in GCF was higher in the case than the control group;
however, the difference was not significant. Further investigations are, therefore,
required to confirm this observation.
