Title of article :
DRE-CALUX bioassay in comparison with HRGC/MS for
measurement of toxic equivalence in environmental samples
Author/Authors :
K.E. Joung a، نويسنده , , b، نويسنده , , Y.H. Chung، نويسنده , , c، نويسنده , , Y.Y. Sheen a، نويسنده , , ?، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
CALUX, Chemically Activated LUciferase gene eXpression bioassay, has proven valuable for screening for and assessing toxic
equivalents of dioxin-like compounds, because it detects all AhR (arylhydrocarbon receptor) ligands in a variety of sample
matrices. In this study, we tried to validate DRE (dioxin-response elements)-CALUX bioassay, which has been developed by
cloning mouse cyp1a1 gene in front of luciferase reporter gene. We compared DRE-CALUX bioassay with high resolution gas
chromatography/mass spectrometry (HRGC/MS) for assessing environmental samples from Korea. 2,3,7,8-tetrachlorodibenzo-pdioxin
(TCDD) dose response study showed high correlation (r2=0.89) between DRE-CALUX bioassay and EROD
(ethoxyresorufin O-deethylase) bioassay, a commonly used bioassay method. The mean TEQ value of water samples was
0.57 pg-TEQHRGC/MS/L and 4.97 pg-TEQCALUX/L. For soil samples, HRGC/MS-TEQ values ranged from 0 to 47.18 pg-TEQ/g
(dry) and correlated well (r2=0.98) with values obtained by CALUX-TEQ which ranged from 0.92 to 649.97 pg-TEQ/g (dry). The
difference between the absolute TEQ values might be due to the presence of dioxin-like compounds without WHO-TEQ values
rather than the difference between CALUX-REP and WHO-TEQ. Based on this study, we suggest that DRE-CALUX bioassay can
serve as an alternative bioassay method for high-throughput analysis of large number of environmental samples.
Keywords :
dioxin , TEQ , EROD , HRGC/MS , CALUX
Journal title :
Science of the Total Environment
Journal title :
Science of the Total Environment