Title of article :
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone is correlated with 8-hydroxy-2′-deoxyguanosine in humans after exposure to environmental tobacco smoke Original Research Article
Author/Authors :
Hung-Che Chiang، نويسنده , , Yung-Kai Huang، نويسنده , , Pei-Fen Chen، نويسنده , , Chia-chi Chang، نويسنده , , Chien-Jen Wang، نويسنده , , Pinpin Lin، نويسنده , , Hui-Ling Lee، نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2012
Pages :
6
From page :
134
To page :
139
Abstract :
Cigarette smoking and exposure to environmental tobacco smoke (ETS) are important risk factors for many cancers. However, exposure doses have usually not been quantitatively assessed in human studies. In humans 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronate conjugate (defined as total NNAL) are the major metabolites of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a cigarette-specific carcinogen. Although animal studies have shown that exposure to cigarette smoke increases tissue oxidative DNA damage, the relationship between cigarette smoke and 8-hydroxydeoxyguanosine (8-OHdG) is not consistent in human studies. In the present study, we have developed a simple, sensitive, and robust LC–MS/MS method for quantifying total NNAL and 8-OHdG concentrations in human plasma. We quantified total NNAL and 8-OHdG in plasma as well as 8-OHdG in urine of 121 healthy male subjects. Total NNAL levels were significantly higher in ever-smokers than in never-smokers. Furthermore, total NNAL levels in plasma were increased with numbers of cigarettes smoked per day in ever-smokers. It suggests that total NNAL in plasma is a good biomarker for cigarette smoke exposure. After stratifying by smoking status and adjusting for age, ETS exposure and occupation category, total NNAL was associated with plasma and urinary 8-OHdG in never-smokers, but not in ever-smokers. Since total NNAL levels in nonsmokers represented the ETS exposure, it appears that 8-OHdG levels are dose-dependently correlated with their ETS exposure dose. Furthermore, this correlation supports the hypothesis that oxidative DNA damage is one of major adverse effects induced by ETS exposure in humans.
Keywords :
Environmental tobacco smoke , 8-hydroxydeoxyguanosine , 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone , LC–MS/MS
Journal title :
Science of the Total Environment
Serial Year :
2012
Journal title :
Science of the Total Environment
Record number :
987996
Link To Document :
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