Title of article :
A new approach for the quantification of root-cap mucilage exudation in the soil
Author/Authors :
Iijima، M. نويسنده , , Sako، Y. نويسنده , , Rao، T. P. نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Pages :
-398
From page :
399
To page :
0
Abstract :
Direct evidence on the functions of root-cap mucilage during plant root growth in soil is limited mainly due to the lack of a method for in situ measurements. In this paper, we offer a method that facilitates the measurement of mucilage exudation when roots are growing in soil. We observed the mucilage exudation directly through a transparent panel located on the side of a root box in which plant roots were growing. We used a CCD camera attached to a microscope to observe and record mucilage exudation. Using image analysis, the activity of mucilage exudation was evaluated based on the area occupied by the mucilage on the root tip. The area of mucilage observed on the root tips after 1-h growth in soil corresponded with the weight of mucilage that was originally observed on the tips before they were transplanted. This relationship suggests that the observed area on root tip relates to total exudation. The area of mucilage exudation on the root tips was high (0.48 mm^2) at night and low (0.35 mm2) at midday, suggesting that the activity of mucilage exudation follows diurnal changes. Furthermore, the mucilage exudation positively correlated with the root elongation rate, implying that fast-growing roots exude more mucilage.
Keywords :
CCD camera , image analysis , diurnal changes , maize , root-cap , mucilage
Journal title :
PLANT AND SOIL
Serial Year :
2003
Journal title :
PLANT AND SOIL
Record number :
98892
Link To Document :
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