Effect of electroporation conditions on cell viability of Frankia EuI1c

The feasibility of electrotransformation of Frankia strain EuI1c was investigated. Cell viability decreased as the capacitance of the electrical pulse increased. The addition of 10% glycerol to the electroporation buffer increased cell viability rates. At a fixed capacitance, cell viability was voltage-dependent and resistance-dependent. Electroporation with a high capacitance was mutagenic and produced antibiotic-resistant cells. Electroporation of Frankia strain EAN1pec chromosomal DNA into strain EuI1c generated several colonies that were resistant to lincomycin or kasugamycin.