Photoluminescence properties of morin and lysozyme molecules absorbed on anodic porous alumina membrane

For the first time, an organic dye, morin, and bio-macromolecule, lysozyme (Lys) were embedded in the holes of anodic porous alumina (P-Al). The photoluminescent (PL) spectra of dye and dye–protein immersed in P-Al film were investigated and compared with those of liquid solutions. It was found that their PL positions are similar to that of dye–Al3þ in ethanol solution, and the PL intensity of embedded dye can be enhanced greatly by the introduction of lysozyme.We ascribe that the appearance of the PL band detected is attributed to the formation of morin–Al complex in the pores with its inner wall involved. A likely orderly model was proposed to explain the observed enhancing phenomena of PL due to the coexistence of morin and lysozyme in the P-Al layer, which has been proved by UV and FTIR measurements. # 2004 Elsevier B.V. All rights reserved