شناسه هاي افزوده :
معصوم زاده ، مهدي ، همكارطرح , عليزاده ،مهدي ، همكارطرح , بازاري مقدم ، سهيل ، همكارطرح , شناور ماسوله،عليرضا ، همكارطرح , مهدي نژاد، كريم ، همكارطرح , حلاجيان، علي ، همكارطرح , قاسمي، محدث ، همكارطرح , رمضاني ،بابك ، همكارطرح
چكيده انگليسي :
Abstract
Considering the significance of sturgeon rearing and producing the meat and caviar which are one of the most development programs in aquaculture, it is necessary to investigate on hygiene condition of rearing environment to gather information about current rearing condition and to adopt the best method for prevention of disease and treatment in sturgeons. This study carried out on 3000 species of farmed Huso huso, before and after transferring to cage, during 7 months from 2011.6.7 to 2012.1.10. Before introducing stock to cage, 100 fingerlings randomly selected and no signs of disease observed. Study on parasites showed that before releasing of fingerlings, Trichodina Sp. found in gill and skin with 40% frequency and mean intensity of 20±1.71. During study in sea environment, no parasites observed in gill, skin and gut. Bacterial investigation showed that total bacteria in rearing water of concert tanks, fish gills and skin is 5.80-5.84 Log cfu ml-1, 3.28-3.41cfug-1 and 5.36-5.58 cfu (cm2)-1. The range of bacterial count in water of sea environment, skin and gill was 3.97-5.92 Log cfu ml-1, 3.74-5.41 cfu (cm2)-1 and 2.01-3.40 cfug-1, respectively. Before and after releasing, the bacteria isolated from fish and rearing water include Enterobacteriaceae, Aeromonas sp., Pseodomonas sp., Acinetobacter sp., Staphylococcus, Halomunas sp. and Shewanella sp. Furthermore, in fungal examinations, the total fungi in rearing water of concert tanks, gill and skin of fingerlings was 12.66-18 cfu ml-1, 2.66-4 cfug-1,5.33-8.66 cfu(cm2)-1, respectively. Fungal count in rearing water of sea environment was 22-52.5 ml-1. It was 13-35.41(cm2)-1 and 8-23.50 cfug-1 in skin and gill.
The fungal flora that isolated from the fish and rearing water, were Cladosporium sp., Penicillium sp., Mucor sp., Aspergilus sp. and Yeasts before and after releasing. Some factors of fish blood serum such as total protein, albumin, blood cholesterol, triglyceride, glucose, magnesium and calcium of the sea water and freshwater determined and compared.
In sea water and fresh water farming conditions, the mean RBC (1102500 ,1067500), WBC (24916.67 , 20625) and White blood cell differential count including Neutrophils (19.41 , 21.25 ), Eosinophils (5.08 ,4) Lymphocytes (72.75, 71.25 ) and Monocytes (2.75 , 3.5) were determined. As well as Hematocrit (20.17, 23.75), Hemoglobin (4.34, 4.27), MCV (783.06, 22.79), MCH (39.42, 39.43) and MCHC (21.69, 17.77) Calculated. The fish blood serum factors including Total protein (1.80, 2.89), Albumin (0.55, 7.89), Cholesterol (70.16, 76.92), Triglyceride (615.74, 330.04), Glucose (64.32, 51.90), Magnesium (9.12, 9.51) and Calcium (5.37, 14.84) were determined and compared in sea water and fresh water, respectively. According to results, significant differences observed in albumin and calcium rate in fishes of freshwater and triglyceride in fishes of sea water (P<0.05). Moreover, the mentioned serum factors, showed significant differences during various months (P<0.05). Examination of 240 fishes showed 10.41% of them had hemorrhage in scutes. Also, investigation on internal organs in reared fish after autopsy showed 40% of them had degeneration of fatty liver. Generally, despite occurrence of some skin sores and fatty liver in the sampled fishes, no infectious disease that led to mortality have not been observed and the fishes which reared in cage had relatively satisfactory health
