چكيده لاتين :
An on-line UV-HPLC / APCI-MS (atmospheric pressure chemical ionization) tandem by a platinium Ci 8 column with an isocratic solvent system after optimizing was applied to the separation and the identification ofdifferent carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. This method was used to identify particular carotenoidsproducedby newlyisolatedmicroorganismstrain ofSporobolomyces ruberrimus H110 in our laboratory. The developed method allows to distinguish torularhodin and canthaxanthin, having the same molar mass but a different chemical structure. A detection limit of 9ng /ml for torularhodin observed. CV (%) for measurement ofall carotenoid concentration was always below 3 %. Torularhodin and for a smaller part beta carotene were the two pigments identified in Sporobolmyces ruberrimus H110.
