چكيده لاتين :
Many modern analytical methods deal with the trace-level determination of
compounds of interest in highly complex environmental samples by means of chromatographic
techniques. The introduction ofa "clean" sample into an analytical instrument can make analyses
easier and prolongs the equipment life. The use ofsolid-phase extraction (SPE) has grown and is a
fertile technique ofsample preparation as it provides better results than those produced by liquidliquid
extraction (LLE). The application of SPE can give selectivity of extraction providing a
purified and concentrated extract. Through this study, optimization oftrace enrichment and sample
clean-up method via the use of bonded silica cartridges is discussed SPE using bonded silica has
been optimized with respect of sample pH, sample concentration, elution solvent strength, sample
volume, and elution volume. In this investigation a variety of non-polar sorbent cartridges were
also screened During this study, the octadecyl bonded silica cartridge (C18) has proven successful
in simplifying sample preparation. The present approach proved that 2,4-D could be retained on
C18 based on specific interaction. Further study employed methanol to extract the analyte from
spiked water and gave a clean sample for high pressure liquid chromatography equipped with ultra
violet detection system. The optimized method was validated with three different pools of spiked
samples and showed good reproducibility over six consecutive days as well as six within-day
experiments
