شماره ركورد :
14013
عنوان به زبان ديگر :
Immunocytochemical Study on Microtubule Reorganization in HL-60 Leukemia Cells Undergoing Apoptosis
پديد آورندگان :
Ahmadian Shahin نويسنده , Pazhang Yaghub نويسنده , Shariftabrizi Ahmad نويسنده
از صفحه :
209
تا صفحه :
214
تعداد صفحه :
6
چكيده لاتين :
Background: Microtubules (MT) are in1portant components of cell cytoskeleton and play key roles in cell motility n1itosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic drugs. The aim of this study was to correlate the changes in the MT with behavior of the y-tubulin in apoptotic cell, and to see if apoptitic MT showed biochemical characteristics of stable MT. Methods: Apoptosis was induced in the human leukemia cells (HL-60) by treatment with 1 JiM of all-trans retinoic acid over a 5-day period. The time course of changes was assessed using flow cytometry, DNA fragmentation and immunocytochen1istry in cells labeled for a-tubulins, acetylated a-tubulin and y-tubulin. Results: The results indicated that y-tubulin content is increased after cells have gone through the apoptosis with a diffuse cytoplasmic pattern. a-tubulin did not reveal any specific pattern of polymerization in apoptotic cells and acetylated a-tubulin content was also decreased in comparison with non-apoptotic cells. Conclusion: Our results support the idea that microtubule reorganization is an important factor of the mammalian cells response to apoptosis, and the altered properties of the MT did not reflect changes in function as apoptosis progresses. Iran. Biomed. J. 11 (4): 209-214,2007
شماره مدرك :
1197704
لينک به اين مدرک :
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