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Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore

Shahverdi Abdolhossein نويسنده , Movahedin Mansoureh نويسنده , Rezazadeh Valojerdi Mojtaba نويسنده , Kazemi Ashtiani Saeid نويسنده

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250

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Background: The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (Cal) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without Cal-treated sperms. Results: Piezo-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, PO.001) and a cleavage rate of 73% (conventional ICSI: 58%, PO.05). When the Piezo-ICSI procedure was performed with Cal-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, PO.05). However, the fertilization rate did not change significantly (64% vs. 56%). Conclusion: The Piezo-ICSI accompanies with Cal-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos. Iran. Biomed. J. 11 (4): 245-250, 2007

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