پديد آورندگان :
Shaiegan Mojgan نويسنده , Pourfatollah Ali-Akbar نويسنده , Namiri Mehrnaz نويسنده , Babaee Gholan1reza نويسنده
چكيده لاتين :
Platelet transfusion is accompanied by febrile nonhemolytic transfusion
reactions. The generation of cytokines (like IL-1 beta, IL-6, IL-8, and TNF-alpha) in platelet
concentrates by white cells is suggested to be responsible for febrile nonhemolytic transfusion
reactions. The number of white cells in the platelet concentrates is crucial to cytokine generation.
Methods: This study was performed to determine whether WBC reduction in platelet
concentrates by prestorage leukodepletion filters or inactivation by gamma radiation reduced the
levels of these cytokines during storage for 3 days. Each of the platelet concentrates (n = 54) was
prepared from a single random donor by platelet-rich plasma. This was then divided into four
groups: 1) unfiltered, nonirradiated random-donner platelet concentrates (n = 13); 2) unfiltered,
gamma-irradiated random-donner platelet concentrates (n =16); 3) filtered, nonirradiated randomdonner
platelet concentrates (n =14); and 4) filtered, gamma-irradiated random-donner platelet
concentrates (n = 11).
Cytokine levels in platelet concentrates supernatants were measured by ELISA kits according
to the manufacturerיs recommendations.
Results: Our results showed that IL-8 was detected in unfiltered, nonirradiated, and gammairradiated
random-donner platelet concentrates but not in the filtered random-donner platelet
concentrates. TNF-alpha was only detected in unfiltered, nonirradiated units. Compared with
unfiltered platelet concentrates, prestorage filtration prevented a rise in the IL-8 and TNF-alpha on
day 3 of storage. The concentration of IL-1 beta was lower than the minimum concentration value
of the kit used for this purpose. IL-6 was detected only in 7 units of all filtered platelet concentrates
on day 3.
Conclusion: These data indicate that gamma irradiation can not prevent generation of IL-8 in
platelet concentrates during storage, but prestorage leukoreduction of platelet concentrates can
prevent accumulation of IL-6, IL-8, and TNF-alpha during storage.
