چكيده لاتين :
Background and purpose of the study: There is no report on the effect of seeding-medium
ingredients on Saccharopolyspora erythraea growth and erythromycin production. In this
study, the enhancing effects of seeding-media which have been used routinely for
screening, isolation or identification of actinomycetes, on Saccharopolyspora erythraea
growth and erythromycin production were investigated.
Methods: The control medium contained soybean meal, glucose, glycerol, (NH4)2SO4 and
CaCO3 as the major constituents and several media (I, II, III, ISP2, ISP3 and ISP4) were
used for production of the antibiotic. Concentrations of biomass and erythromycin were
measured by spectrophotometry, HPLC and bioassay methods and the effects of the
composition of seeding media on pH were also determined.
Results: The concentration of erythromycin in medium II was 2.71g/l (1.33 times more than
that of the control medium) and trends in production were: medium II > ISP4 = ISP2 =
Control > ISP2 = Control = ISP3 > ISP3 = medium III = medium I. In the media containing
starch and casein, the hyphae of S. erythraea were star-shaped and much branched.
Major conclusion: By virtue of other technical and economical characteristics of the
seeding media used for erythromycin production, it is concluded that medium II is the best
seeding medium formulation for erythromycin in comparison to others.
