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Molecular typing of avian Pasteurella multocida isolates by PCR-RFLP of ompH gene

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Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification using EcoRI, Hind111 and Cfol endonucleases produced 7 different patterns for the twenty five isolates of the four Pmultocida serotypes. The PCR-RFLP of the ompH gene was found to be potentially a useful method for typing of P. multocida and therefore, for studying the epidemiology of P. multocida infections.

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