مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Background: In vitro maturation (IVM) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). The pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization ofIVM remains a challenge. Objective: In this study, we investigated the effect of cumulus cells on maturation and fertilization rate of immature oocytes (Germinal vesicle). Materials and Methods: Germinal vesicle (GV) oocytes were recovered from 6-8 weeks old Balb C female mice 48hr after injection of 10 IV pregnant mare serum gonadotropin (PMSG). Collected oocytes were divided into two groups. Group A: GV oocytes without cumulus (denuded oocyte). Group B: GV oocytes with cumulus cells (cumulus-oocyte complex) . The oocytes in both groups were cultured in TCM-199 medium in a humidified atmosphere 01 .)/ 0 ( ! ) 2 in air at 3ic. The maturation, fertilization and developme tal rates were recorded יtfter 24hr. Results: Maturation, fertilization and developmental rates in ( nuded oocytos (DO) were 65.1%, 68.02 %, 78.63% respectively, and in cumulus-oocyte complex (COC) were 78.20%, 85.57% and 85.05%, respectively. The maturation, fertilization and d velopmental rates of cae were significantly higher than those ofDO (p<0.05). Conclusion: The results show that cumulus cells have beneficial effect on maturation, fertilization and cleavage rates of mice oocytes.