پديد آورندگان :
Nayemia Karim نويسنده , Lee Jae Ho نويسنده , Engel Wolfgang نويسنده , Drusenheime Nadja نويسنده , Rathsack Kristina نويسنده , Dev Arvind نويسنده , Wulf Gerald نويسنده , Ehrmann Ingrid E. نويسنده , Elliot David نويسنده , Zechnel Ulrich نويسنده , Haal Thomas نويسنده , Meinhard Andreas نويسنده , Michelmann HansW. نويسنده , Hasenfuss Gerlad نويسنده , Guan Kaomei نويسنده
چكيده لاتين :
Germline and somatic stem cells are distinct types of stem cells that are dedicated to
reproduction and somatic tissue regeneration, respectively. Germline stem cells (GSCs),
which can self-renew and generate gametes, are unique stem cells in that they are solely
dedicated to transmit genetic information from generation to generation. We developed
a strategy for the establishment of germline stem cell lines from embryonic stem cells
(ES) . These cells are able to undergo meiosis, generate haploid male gametes in vitro
and are functio nal, as shown by fertilization after intra-cytoplasmic injection into mouse
oocytes. In other approach, we show that bone marrow stem (BMS) cells are able to
trans-differentiate into male germ cells. BMS cell-derived germ cells expressed the
known molecular markers of primordial germ cells. The ability to derive male germ
cells from ES and BMS cells reveals novel aspects of germ cell development and opens
the possibilities for use of these cells in reproductive medicine. Conversely, we showed
that adult male germline stem cells, spermatogonial stem cells (SSCs), can be converted
into embryonic stem cell like cells which can differentiate into the somatic stem cells of
three germ layers. Understanding how SSC can give rise to pluripotent stem cells and
how somatic stem cells differentiate into germ cells could give significant insights into
the regulation of developmental totipotency as well as having important implications for
male fertility and regenerative medicine.
