چكيده لاتين :
The use of proteins and peptides as hwnan therapeutics has been increased in recent years .
Lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. The
purpose of the present study was to evaluate the effect of hwnidity along with compactional
pressure on the enzymatic activity of wheat germ lipase.
Samples of lipase powder were kept at different relative humidity (RR) conditions (24, 40,
63 and 75 %) before compaction under various pressures (74-372 Mega pascal or Mpa) . The
relative enzymatic activity of the compacts was then determined by titration method using
Triacetin as substrate. Density measurements were also conducted in order to describe the
possible mechanism of the enzymatic activity. The results indicated densities of the compacts
prepared under various compactional pressures increase as humidity rises. Based on the results ,
activity loss of the compacts following relative humidity increase can be related to steric
hindrance caused by higher pressures. However, there was no significant difference between
densities at different compactional pressures at a given hwnidity condition.
