17192

Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

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A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil Cs column (l50x4 mm) and a UV detector (11,= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml minי was used as mobile phase. The proteins were precipitated with an acetonitrile solution containing diltiazem as internal standard and the average recovery was 93.9±3.4%. The detection limit for risperidone in plasma was 0.5 ngml". The calibration curve was linear over the concentration range 2-50 ngmlי. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of risperidone in human subjects.

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