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Isolation and Identification of Calystegines in Root Cultures of four Physalis Species

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Calystegines were isolated from the root cultures of Physalis divaricata, P pubescens, P philadelphia,and P philadelphia (solanaceae). Calystegines of P divaricata were identified as calystegine A3, Asי BI and B2. with concentrations of 6.99, 4.41, 8.52, and 14.7 ug/g in fresh mass, respectively. Root cultures of Ppubescens contain calystegine A3, B1 and B2, P philadelphia only contains calystegine B2 and P philadelphia root cultures solely consist of calystegineAy Isolation and identification of calystegines have been achieved by ion exchange column chromatography and gas chromatography, using authentic samples.

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