مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Vancomycin (glycopeptide)-resistant enterococci (VRE or GRE) can cause serious problems for hospitalized patients due to the limited options for treatment of VRE infections. As infection with VRE increases in hospitals, further knowledge about vancomycin resistant genes is needed. Methods: Isolates of Enterococcus spp . were collected from hospitalized patients in Tehran (Iran) during 2006. Detailed molecular anal ysis was performed for vancomycin resistance genotype and vanHAX using con ventional PCR and PCRRFLP (re striction fragment length polymorphism), respectively. Results: out of 830 enterococci spp., 48 VRE isolates (5 .8%) wer e obtained. All of VRE isolates carried vanA gen e. DdeI digestion of vanHAX element showed the presence of point mutation at 8234 position. Conclusion: Thi s study indicates that vanA is a predominant genotype in Iranian isolates . In addition, PCR-RFLP analysis revealed the presence of two types of vanHAXelement in vanA harboring transposons. Iran. Biomed. 1. 12 (4) : 223-228, 2008