چكيده لاتين :
Titanium (Ti) is a relatively abundant element that has found growing applications in medical science
and recently some of Ti compounds are introduced as anticancer drugs. In spite of very limited data
which exist on the Ti metabolism, some proteins might be involved in the mechanism of action of Ti.
Up to our knowledge, there is not any report in the literature concerning binding of Ti to
apolactoferrin. Binding of apolactoferrin with Ti(lV)-citrate was studied by spectroflourimeterey and
spectrophotometery techniques under physiological conditions. The spectroflourimeteric studies
revealed a significant fluorescence quenching, that indicated binding of apolactoferrin with Ti(lV).
The same reaction was monitored through spectrophotometry technique; this represents a
characteristic UV difference band at 267 nm, which is different from lac-Fe (III). Titration studies
show that lactoferrin specifically binds two moles Ti(lV) as complex with citrate per mol protein.
Spectroflourimeterey and spectrophotometery techniques indicated that Ti(lV) ions cause a reduction
(13%-14%) in binding of Fe(lll) to lactoferrin. In overall, we may come to this conclusion that this
element might be involved in the iron metabolism. Iran. Biomed. 1. 10 (2): 93-98, 2006
